Algal growth and spreading are important factors that determine the
magnitude and location of HAB events. These factors drive the production
and movement of marine toxins through the marine food web. In order
to understand how biotoxins move through the food web, we have several
ongoing projects investigating the individual components of HAB
events:
Blooms of
Pseudo-nitzschia, the organism capable of producing
domoic acid, occur sporadically along the West Coast of North America.
How and why these blooms occur is not understood. A predictive model
explaining these occurrences would provide risk managers with a powerful
tool in mitigating domoic acid impact on marine resources. Our current
working hypothesis is that
Pseudo-nitzschia bloom in waters over
the Washington continental shelf in an oceanic structure called the
Juan De Fuca eddy, a retentive feature that is thought to be an "initiation
site" of these blooms. We suspect that this location may be the
"breeding ground" of
Pseudo-nitschia and that the
se
diatoms are transported from this area to the beaches where they
become responsible for domoic acid accumulation in razor clams. The
precise timing of these physical processes (currents, winds, upwelling,
and downwelling) determines whether a bloom will be advected into the
nearshore region and be sustained there long enough for razor clams
to become toxic.
There are several species of
Pseudo-nitschia and not all species
produce toxin. Which ones produce the toxins and under what conditions
is not completely understood. To address these questions the cells are
isolated from a sample and identified using SEM and genetic probes.
Once the species has been identified they are grown in culture under
differing nutrient conditions. Toxin production is then measured by
the receptor binding assay.