Validation of ELISA Field Kits

Recently, some kits have been developed that can detect marine biotoxins in shellfish and phytoplankton/seawater. In using these kits, samples of shellfish or phytoplankton (collected on a filter) are extracted and then the extract is applied to a small cartridge. The cartridge is allowed to “develop”, usually about 5 minutes, and then the presence or absence of two colored lines is observed. If only one line (the “control”) is seen then toxin is present in the sample. If two lines are observed, then toxin is considered absent.

This technology, when applied as a screening device, would be useful if it were implemented in a monitoring and surveillance program. These kits could be used in the field to screen for the presence or absence of toxins. Used as a screen, the kits would reduce the number of samples that needed to be sent to state testing labs, thereby reducing costs and/or resources for surveillance programs.

 

 

 

 

 

 

 

 

 

 

 

 

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