NOAA/NMFS/NWFSC/TM14: P-Postlabeling Protocols for Assaying Levels of Hydrophobic DNA Adducts in Fish

Table 1. Typical Excel worksheet showing Phosphoimager data and calculation of DNA damage
PPL#	Sample #	Study	Site	Species	PI vol-a	CF-a	cpm-a	SA-a	AQ-a	PI vol-b	CF-b	CPM-b	SA-b	AQ-b	dG-CF	dil-b	nmol-b	fmol-a	nmol/mol-b
							** F2/G2					K2/L2					(M2O2P2*Q2) /N2	(H2*J2) /I2	S2/ (R2*0.001)
148-01	90.3180	R&F '90	Pristine Sound	P. Mudgrunt	951268	8060	118	1939	2	4024071	126	31836	968055	2.4	4.76	100	38	0.12	3
148-02	90.3181	R&F '90	Pristine Sound	P. Mudgrunt	718964	8060	89	1939	2	4761861	126	37673	968055	2.4	4.76	100	44	0.09	2
148-03	90.3182	R&F '90	Pristine Sound	P. Mudgrunt	499168	8060	62	1939	2	2406825	126	19041	968055	2.4	4.76	100	22	0.06	3
148-04	90.3183	R&F '90	Pristine Sound	P. Mudgrunt	1133085	8060	141	1939	2	2993307	126	23681	968055	2.4	4.76	100	28	0.15	5
148-05	90.3184	R&F '90	Pristine Sound	P. Mudgrunt	572750	8060	71	1939	2	2721056	126	21527	968055	2.4	4.76	100	25	0.07	3
148-06	90.3185	R&F '90	Pristine Sound	P. Mudgrunt	581683	8060	72	1939	2	1472010	126	11646	968055	2.4	4.76	100	14	0.07	5
148-07	90.3186	R&F '90	Pristine Sound	P. Mudgrunt	1003146	8060	124	1939	2	2168595	126	17157	968055	2.4	4.76	100	20	0.13	6
148-08	90.3187	R&F '90	Pristine Sound	P. Mudgrunt	1562322	8060	194	1939	2	4421854	126	34983	968055	2.4	4.76	100	41	0.20	5
148-09	91.9980	R&F '91	Urban Bay	P. Mudgrunt	10035754	8060	1245	1939	2	2093496	126	16562	968055	2.4	4.76	100	20	1.28	66
148-10	91.9981	R&F '91	Urban Bay	P. Mudgrunt	32846867	8060	4075	1939	2	3719354	126	29425	968055	2.4	4.76	100	35	4.20	121
148-11	91.9982	R&F '91	Urban Bay	P. Mudgrunt	29776508	8060	3694	1939	2	3929672	126	31089	968055	2.4	4.76	100	37	3.81	104
148-12	91.9983	R&F '91	Urban Bay	P. Mudgrunt	33118195	8060	4109	1939	2	2539131	126	20088	968055	2.4	4.76	100	24	4.24	179
148-13	91.9984	R&F '91	Urban Bay	P. Mudgrunt	45305225	8060	5621	1939	2	3358298	126	26569	968055	2.4	4.76	100	31	5.80	185
148-14	91.9985	R&F '91	Urban Bay	P. Mudgrunt	97089089	8060	12046	1939	2	4359981	126	34494	968055	2.4	4.76	100	41	12.42	305
148-15	91.9986	R&F '91	Urban Bay	Sp. Mudgrunt	4077333	8060	506	1939	2	3052623	126	24150	968055	2.4	4.76	100	28	0.52	18
148-16	91.9987	R&F '91	Urban Bay	Sp. Mudgrunt	336385	8060	42	1939	2	1089497	126	8619	968055	2.4	4.76	100	10	0.04	4
148-17	91.9988	R&F '91	Urban Bay	Sp. Mudgrunt	2638708	6852	385	1939	2	3208020	126	25380	968055	2.4	4.76	100	30	0.40	13
148-18	91.9989	R&F '91	Urban Bay	Sp. Mudgrunt	1494894	6852	218	1939	2	2414327	126	19101	968055	2.4	4.76	100	23	0.22	10
148-19	91.9990	R&F '91	Urban Bay	Sp. Mudgrunt	734904	6852	107	1939	2	2715641	126	21485	968055	2.4	4.76	100	25	0.11	4
148-20	91.9991	R&F '91	Urban Bay	Sp. Mudgrunt	1733913	6852	253	1939	2	3768276	126	29812	968055	2.4	4.76	100	35	0.26	7
148-21	91.9992	R&F '91	Urban Bay	Sp. Mudgrunt	2072451	6852	302	1939	2	2628056	126	20792	968055	2.4	4.76	100	25	0.31	13
BP					34783296	8314	4184	1939	1.333									2.88

Table 1. Typical Excel worksheet showing Phosphoimager data and calculation of DNA damage