Contaminant Exposure in Juvenile Salmon: Appendix Table 1

U.S. Dept Commerce/NOAA/NMFS/NWFSC/Publications

NOAA Tech Memo NMFS NWFSC-8: Contaminant Exposure in Juvenile Salmon (cont):
Appendix Table 1

Table A-1.
Concentrations, ng/g (ppb) wet weight, of aromatic contaminants in stomach contents of juvenile chinook salmon sampled in 1989. - a,b,c,d,e,f,g,j


Site name

Green River Hatchery


Duwamish River estuary

Nisqually Hatchery


Nisqually River estuary


Puyallup Hatchery
Date collected
5/8/89
5/23/89
6/1/89
6/7/89
5/22/89
6/12/89
6/20/89
6/27/89
7/7/89
5/24/89
Aromatic contaminantsLab no.
63-283
63-279
63-280
63-281
63-284
63-285
63-336
63-337
63-338
63-286

naphthalene

17


26


1100


21


21


29


19


6


15


12
C1-naphthalenes
2
19
9800
29
23
5
7
5
7
17
C2-naphthalenes
< 0.7
11
37000
16
21
< 0.6
< 0.2
7
< 0.3
< 0.2
C3-naphthalenes
< 0.7
2
55000
0.6
7
< 0.6
< 0.2
3
< 0.3
< 0.2
C4-naphthalenes
< 0.7
< 0.4
40000
< 0.4
< 0.1
< 0.6
< 0.2
0.6
< 0.3
< 0.2
acenaphthylene
< 1
22
510
< 0.6
0.5
< 1
< 0.3
< 0.2
< 0.5
< 0.3
acenaphthene
< 2
65
1400
100
< 0.2
< 1
3
< 0.2
< 0.7
< 0.4
fluorene
< 2
82
33
170
2
< 1
< 0.4
< 0.2
< 0.6
< 0.4
C1-fluorenes
< 2
9
17000
< 0.8
< 0.2
< 1
< 0.4
< 0.2
< 0.6
< 0.4
C2-fluorenes
< 2
3
34000
< 0.8
< 0.2
< 1
< 0.4
< 0.2
< 0.6
< 0.4
C3-fluorenes
< 2
1
16000
< 0.8
< 0.2
< 1
< 0.4
1
< 0.6
< 0.4
phenanthrene
4
550
11000
850
29
11
3
4
5
11
C1-phenanthrenes/anthracenes
< 0.7
30
26000
< 0.3
19
< 0.6
< 0.2
2
< 0.3
< 0.2
C2-phenanthrenes/anthracenes
< 0.7
580
23000
200
14
< 0.6
< 0.2
3
< 0.3
< 0.2
C3-phenanthrenes/anthracenes
< 0.7
220
4700
56
< 0.1
< 0.6
< 0.2
0.5
< 0.3
< 0.2
C4-phenanthrenes/anthracenes
< 0.7
160
1800
< 0.3
< 0.1
< 0.6
< 0.2
< 0.09
< 0.3
< 0.2
dibenzothiophene
< 1
29
9500
79
0.7
< 0.8
< 0.3
0.3
< 0.4
< 0.2
C1-dibenzothiophenes
< 1
15
23000
56
< 0.1
< 0.8
< 0.3
< 0.1
< 0.4
< 0.2
C2-dibenzothiophenes
< 1
41
29000
94
< 0.1
< 0.8
< 0.3
< 0.1
< 0.4
< 0.2
C3-dibenzothiophenes
< 1
11
17000
93
< 0.1
< 0.8
< 0.3
< 0.1
< 0.4
< 0.2
Sum of LACs
23
1900
360000
1800
140
45
32
32
27
40
fluoranthene
< 0.8
11000
500
1700
9
< 0.7
1
3
0.9
0.8
pyrene
< 0.7
15000
91
990
8
< 0.6
1
4
0.6
1
C1-fluoranthenes/pyrenes
< 0.8
4300
2900
250
3
< 0.7
< 0.2
< 0.1
< 0.4
< 0.2
benz[a]anthracene
< 0.8
3300
910
250
0.7
< 0.7
< 0.3
< 0.1
< 0.4
< 0.2
chrysene
< 0.6
7500
1600
260
2
< 0.5
< 0.2
0.9
< 0.3
< 0.1
C1-chrysenes/benz[a]anthracenes
< 0.6
900
< 0.1
38
< 0.09
< 0.5
< 0.2
< 0.09
< 0.3
< 0.1
C2-chrysenes/benz[a]anthracenes
< 0.6
180
330
< 0.3
< 0.09
< 0.5
< 0.2
< 0.09
< 0.3
< 0.1
C3-chrysenes/benz[a]anthracenes
< 0.6
16
< 0.1
< 0.3
< 0.09
< 0.5
< 0.2
< 0.09
< 0.3
< 0.1
C4-chrysenes/benz[a]anthracenes
< 0.6
32
< 0.1
< 0.3
< 0.09
< 0.5
< 0.2
< 0.09
< 0.3
< 0.1
benzo[b]fluoranthene
< 0.6
4600
780
170
< 0.09
< 0.6
< 0.2
< 0.1
< 0.4
< 0.1
benzo[k]fluoranthenei
< 0.6
2000
320
71
< 0.08
< 0.5
< 0.2
< 0.08
< 0.3
< 0.1
benzo[a]pyrene
< 0.6
180
240
56
0.3
< 0.5
< 0.2
< 0.09
< 0.3
< 0.1
indeno[1,2,3-cd]pyrene
< 0.8
1500
320
24
< 0.1
< 0.7
< 0.3
< 0.1
< 0.4
< 0.2
dibenz[a,h]anthracene
< 0.9
83
35
2
< 0.1
< 0.8
< 0.2
< 0.1
< 0.4
< 0.2
benzo[ghi]perylene
< 0.6
1200
250
21
< 0.09
< 0.5
< 0.2
< 0.08
< 0.3
< 0.1
Sum of HACs
-
52000
8300
3800
23
-
2
8
2
2
Sample weight, grams
0.51
0.80
1.27
0.75
2.46
0.57
1.01
3.01
1.11
1.68

Footnotes follow

Table A-1. Continued.
Concentrations, ng/g (ppb) wet weight, of aromatic contaminants in stomach contents of juvenile chinook salmon sampled in 1989. - a,b,c,d,e,f,g,j


Site name

Puyallup Hatchery


Commencement Bay


Skykomish Hatchery


Snohomish River estuaryh
Date collected
5/31/89
5/31/89
6/14/89
6/19/89
6/26/89
5/30/89
6/6/89
6/22/89
6/28/89
6/29/89
Aromatic contaminantsLab no.
63-286k
63-287k
63-339
63-340
63-341
63-299
63-300
63-342
63-343
63-344

naphthalene

10


12


21


24


55


23


21


[39]


[96]


15
C1-naphthalenes
9
17
56
40
110
26
37
[17]
[31]
6
C2-naphthalenes
< 0.6
< 0.1
270
110
170
9
18
[31]
[30]
5
C3-naphthalenes
< 0.6
< 0.1
550
170
210
< 0.2
< 0.3
[12]
< 2
< 0.3
C4-naphthalenes
< 0.6
< 0.1
510
220
190
< 0.2
< 0.3
[6]
[2]
< 0.3
acenaphthylene
< 0.9
< 0.2
14
4
11
< 0.2
< 0.5
< 2
< 3
< 0.5
acenaphthene
< 1
< 0.3
4
16
100
< 0.3
< 0.7
< 2
[56]
3
fluorene
< 1
< 0.3
110
28
170
< 0.3
< 0.7
[3]
[90]
2
C1-fluorenes
< 1
< 0.3
170
74
83
< 0.3
< 0.7
[7]
[20]
< 0.6
C2-fluorenes
< 1
< 0.3
440
330
220
< 0.3
< 0.7
< 2
[45]
7
C3-fluorenes
< 1
< 0.3
190
180
110
< 0.3
< 0.7
[16]
< 4
< 0.6
phenanthrene
1
5
470
120
780
11
8
[43]
[600]
15
C1-phenanthrenes/anthracenes
< 0.5
< 0.1
210
150
160
4
< 0.3
[7]
[40]
0.5
C2-phenanthrenes/anthracenes
< 0.5
< 0.1
380
330
270
< 0.1
< 0.3
[19]
[13]
2
C3-phenanthrenes/anthracenes
< 0.5
< 0.1
120
140
130
< 0.1
< 0.3
[4]
[3]
< 0.3
C4-phenanthrenes/anthracenes
< 0.5
< 0.1
65
72
54
< 0.1
< 0.3
< 0.9
< 2
< 0.3
dibenzothiophene
< 0.8
< 0.2
86
40
86
< 0.2
< 0.4
[4]
[45]
0.7
C1-dibenzothiophenes
< 0.8
< 0.2
29
14
14
< 0.2
< 0.4
< 1
< 3
< 0.4
C2-dibenzothiophenes
< 0.8
< 0.2
130
170
95
< 0.2
< 0.4
[4]
< 3
< 0.4
C3-dibenzothiophenes
< 0.8
< 0.2
130
160
100
< 0.2
< 0.4
[6]
< 3
< 0.4
Sum of LACs
20
34
4000
2400
3100
73
84
[220]
[1100]
56
fluoranthene
< 0.6
< 0.2
720
260
1600
0.7
< 0.3
[53]
[340]
17
pyrene
< 0.6
< 0.2
490
220
930
1
< 0.3
[38]
[170]
11
C1-fluoranthenes/pyrenes
< 0.6
< 0.2
160
94
180
< 0.2
< 0.3
[3]
< 2
< 0.3
benz[a]anthracene
< 0.7
< 0.2
230
65
320
< 0.2
< 0.3
[1]
< 2
2
chrysene
< 0.5
< 0.1
400
100
370
< 0.1
< 0.3
[14]
[6]
3
C1-chrysenes/benz[a]anthracenes
< 0.5
< 0.1
120
50
110
< 0.1
< 0.3
[1]
< 2
< 0.3
C2-chrysenes/benz[a]anthracenes
< 0.5
< 0.1
54
80
48
< 0.1
< 0.3
< 0.9
< 2
< 0.3
C3-chrysenes/benz[a]anthracenes
< 0.5
< 0.1
32
77
22
< 0.1
< 0.3
< 0.9
< 2
< 0.3
C4-chrysenes/benz[a]anthracenes
< 0.5
< 0.1
7
5
2
< 0.1
< 0.3
< 0.9
< 2
< 0.3
benzo[b]fluoranthene
< 0.5
< 0.1
260
50
260
< 0.1
< 0.3
[9]
[3]
4
benzo[k]fluoranthenei
< 0.5
< 0.1
200
43
170
< 0.1
< 0.2
[2]
< 2
0.6
benzo[a]pyrene
< 0.5
< 0.1
130
35
90
< 0.1
< 0.2
< 0.9
< 2
< 0.2
indeno[1,2,3-cd]pyrene
< 0.7
< 0.2
120
41
69
< 0.2
< 0.4
< 1
< 2
< 0.3
dibenz[a,h]anthracene
< 0.8
< 0.2
23
5
14
< 0.2
< 0.4
< 1
< 2
< 0.3
benzo[ghi]perylene
< 0.5
< 0.1
98
28
54
< 0.1
< 0.3
< 0.9
< 1
< 0.2
Sum of HACs
-
-
3000
1200
4200
2
-
[120]
[520]
38
Sample weight, grams
1.50
1.94
1.56
0.69
0.90
2.30
1.20
0.28
0.13
0.87

Footnotes follow

Table A-1. Continued.
Concentrations, ng/g (ppb) wet weight, of aromatic contaminants in stomach contents of juvenile chinook salmon sampled in 1989. - a,b,c,d,e,f,g,j


Site name
Snohomish River estuary

Mukilteo Hatchery

Method blanks

NIST reference tissue (Mytilus edulis)l

Previously analyzed NIST reference tissue

(n = 3)
Date collected
5/31/89
7/6/89
Aromatic contaminantsLab no.
63-286
63-301
63-290
63-304
63-326
63-348
63-327
mean
RSDm

naphthalene

13


8


1


3


4


9

3
C1-naphthalenes
7
0.8
< 0.4
< 0.3
3
1
C2-naphthalenes
4
< 0.4
< 0.4
< 0.3
< 0.06
4
C3-naphthalenes
< 0.2
< 0.4
< 0.4
< 0.3
< 0.06
< 0.2
C4-naphthalenes
0.4
< 0.4
< 0.4
< 0.3
< 0.06
< 0.2
acenaphthylene
< 0.4
< 0.6
< 0.6
< 0.4
< 0.09
< 0.4
1
acenaphthene
0.8
< 0.8
< 0.8
< 0.6
< 0.1
< 0.5
0.4
fluorene
1
< 0.8
< 0.8
< 0.6
< 0.1
< 0.5
2
C1-fluorenes
< 0.5
< 0.8
< 0.8
< 0.6
< 0.1
< 0.5
C2-fluorenes
< 0.5
< 0.8
< 0.8
< 0.6
< 0.1
< 0.5
C3-fluorenes
2
< 0.8
< 0.8
< 0.6
< 0.1
< 0.5
phenanthrene
16
35
< 0.3
6
1
1
5
1
28
C1-phenanthrenes/anthracenes
1
34
< 0.3
< 0.3
< 0.06
< 0.2
C2-phenanthrenes/anthracenes
3
46
< 0.3
< 0.3
< 0.06
< 0.2
C3-phenanthrenes/anthracenes
< 0.2
10
< 0.3
< 0.3
< 0.06
< 0.2
C4-phenanthrenes/anthracenes
< 0.2
< 0.3
< 0.3
< 0.3
< 0.06
< 0.2
dibenzothiophene
1
< 0.5
< 0.5
< 0.4
0.3
< 0.3
1
C1-dibenzothiophenes
< 0.3
7
< 0.5
< 0.4
< 0.1
< 0.3
C2-dibenzothiophenes
< 0.3
23
< 0.5
< 0.4
< 0.1
< 0.3
C3-dibenzothiophenes
< 0.3
2
< 0.5
< 0.4
< 0.1
< 0.3
Sum of LACs
49
170
1
9
8
15
fluoranthene
23
74
< 0.4
9
0.6
0.3
39
18
12
pyrene
21
38
< 0.4
6
0.5
< 0.3
37
17
24
C1-fluoranthenes/pyrenes
< 0.3
< 0.4
< 0.4
< 0.3
< 0.06
< 0.3
benz[a]anthracene
2
2
< 0.4
< 0.3
< 0.08
< 0.3
10
chrysene
11
11
< 0.3
< 0.2
< 0.06
< 0.2
20
1
0
C1-chrysenes/benz[a]anthracenes
< 0.2
< 0.3
< 0.3
< 0.2
< 0.06
< 0.2
C2-chrysenes/benz[a]anthracenes
< 0.2
< 0.3
< 0.3
< 0.2
< 0.06
< 0.2
C3-chrysenes/benz[a]anthracenes
< 0.2
< 0.3
< 0.3
< 0.2
< 0.06
< 0.2
C4-chrysenes/benz[a]anthracenes
< 0.2
< 0.3
< 0.3
< 0.2
< 0.06
< 0.2
benzo[b]fluoranthene
0.9
1
< 0.3
< 0.2
0.1
< 0.2
6
benzo[k]fluoranthenei
1
< 0.3
< 0.3
< 0.2
0.09
< 0.2
3
benzo[a]pyrene
< 0.5
< 0.3
< 0.3
< 0.2
0.07
< 0.2
2
indeno[1,2,3-cd]pyrene
< 0.2
< 0.4
< 0.4
< 0.3
< 0.08
< 0.3
0.8
dibenz[a,h]anthracene
< 0.2
< 0.5
< 0.5
< 0.3
< 0.08
< 0.2
< 0.7
benzo[ghi]perylene
< 0.2
< 0.3
< 0.3
< 0.2
< 0.06
< 0.2
2
Sum of HACs
59
130
-
15
1
0.3
Sample weight, grams
1.09
0.99
3.02

Footnotes:

a The concentrations of the analytes and the percent recoveries of the surrogate standards were calculated using hexamethylbenzene as the internal standard.

b The alkylated compounds are not in the calibration solution. The response factors of the parent compound, e.g., naphthalene, phenanthrene are used to calculate the alkylated compounds, e.g., C1, C2, C3, C4 - naphthalenes and C1, C2, C3, C4 - phenanthrenes, respectively.

c Concentrations and initial identifications were determined using gas chromatography/mass spectrometry (GC/MS) - multiple ion detection.

d The "less than" symbol (<) indicates that the analyte was not detected in concentrations above the stated value.

e Low molecular weight aromatic contaminants (LACs) and high molecular weight aromatic contaminants (HACs) are 2,3-ring aromatic compounds and 4,7-ring aromatic compounds, respectively.

f Concentrations less than 10 are rounded to one significant figure; concentrations greater than 10 are rounded to two significant figures.

g Percent recoveries for the internal standards (surrogates) averaged 93%, CV = 16%, n = 81.

h The brackets indicate that due to the very small sample size the values should be considered semiquantitative.

i Benzo[k]fluoranthene and benzo[j]fluoranthene often coelute. This combined concentration is reported.

j The concentration of naphthalene was calculated using the surrogate standard naphthalene-d8; analytes from acenaphthylene through pyrene were calculated using acenaphthene-d10; analytes from benz[a]anthracene through benzo[ghi]perylene were calculated using benzo[a]pyrene-d12. The surrogate standards were used to calculate the concentrations of analytes so that the data can be compared to previously analyzed data.

k These samples are duplicate analyses. The data for the duplicate samples were somewhat different which implies that the samples were not homogeneous.

l Standard reference material from the National Institute of Science and Technology.

m RSD is the relative standard deviation which is the standard deviation divided by the mean and expressed as a percent.


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