Contaminant Exposure in Juvenile Salmon: Apendix Table 2

U.S. Dept Commerce/NOAA/NMFS/NWFSC/Publications

NOAA Tech Memo NMFS NWFSC-8: Contaminant Exposure in Juvenile Salmon (cont): Appendix Table 2

Table A-2.
Concentrations, ng/g (ppb) wet weight, of aromatic contaminants in stomach contents of juvenile chinook salmon sampled in 1990. - ^a,b,c,d,e

Site name
Green River Hatchery
Duwamish River estuary
Nisqually Hatchery
Nisqually River

estuary^g
Puyallup Hatchery

Date collected 5/8/90 5/24/90 6/1/90 6/18/90 5/29/90 6/13/90 6/14/90 6/4/90 6/6/90

Aromatic contaminants Lab no. 63-411 63-413 63-414 63-415 63-417 63-428 63-429 63-418 63-419

naphthalene
26
51
230
2900
60
1
3
21
21

C1-naphthalenes 29 100 920 4200 42 3 5 18 28

C2-naphthalenes 34 120 1600 3500 35 1 2 25 54

C3-naphthalenes 40 170 3500 1900 47 0.8 1 30 53

C4-naphthalenes 43 170 3900 770 10 < 0.2 < 0.2 12 31

acenaphthylene 3 29 12 98 6 0.8 0.8 3 3

acenaphthene 2 76 86 8500 5 0.7 0.9 2 3

fluorene 4 99 220 9100 8 0.8 0.9 4 5

C1-fluorenes 9 75 990 1500 13 < 0.3 < 0.3 5 10

C2-fluorenes 20 210 3000 930 7 < 0.3 < 0.3 15 25

C3-fluorenes < 0.3 240 2800 120 < 2 < 0.3 < 0.3 7 < 0.2

phenanthrene 18 440 870 20000 13 1 2 11 17

C1-phenanthrenes/anthracenes 37 360 2300 4700 11 1 1 15 25

C2-phenanthrenes/anthracenes 50 480 2500 2100 9 1 1 17 30

C3-phenanthrenes/anthracenes 65 380 2400 750 15 0.7 1 7 13

C4-phenanthrenes/anthracenes 17 150 990 210 < 1 < 0.1 < 0.1 < 0.3 8

dibenzothiophene 5 41 270 2300 2 < 0.3 < 0.3 2 4

C1-dibenzothiophenes 14 61 1200 690 3 0.5 0.3 6 12

C2-dibenzothiophenes 19 150 2600 450 3 1 0.4 8 15

C3-dibenzothiophenes 18 150 2300 230 < 1 0.8 0.8 7 13

Sum of LACs 450 3600 33000 65000 290 14 20 220 370

fluoranthene 4 1200 910 17000 7 2 1 6 7

pyrene 10 660 730 9200 7 1 1 6 7

C1-fluoranthenes/pyrenes 25 1200 1100 4900 9 2 0.6 4 7

benz[a]anthracene 2 520 210 2500 1 0.3 0.3 1 2

chrysene 4 1200 320 1900 2 2 0.6 2 3

C1-chrysenes/benz[a]anthracenes 10 340 410 810 1 0.4 0.3 1 5

C2-chrysenes/benz[a]anthracenes 10 100 350 220 < 0.6 < 0.1 < 0.1 < 0.2 1

C3-chrysenes/benz[a]anthracenes 7 36 260 55 < 0.6 < 0.1 < 0.1 < 0.2 1

C4-chrysenes/benz[a]anthracenes 0.6 < 0.2 110 < 0.5 < 0.6 < 0.1 < 0.1 < 0.2 < 0.1

benzo[b]fluoranthene 0.6 360 200 780 1 < 0.1 < 0.1 1 2

benzo[k]fluoranthene^f 0.4 310 130 670 0.8 0.2 0.2 0.8 0.7

benzo[a]pyrene 0.6 150 82 390 0.8 0.2 < 0.1 0.8 0.9

indeno[1,2,3-cd]pyrene 0.3 100 83 160 0.9 0.2 < 0.1 0.7 0.6

dibenz[a,h]anthracene 0.2 30 23 52 0.6 < 0.1 < 0.1 0.2 0.2

benzo[ghi]perylene 0.7 110 150 100 1 0.3 0.2 2 0.6

Sum of HACs 75 6300 5100 39000 32 9 4 26 38

Sample weight, grams 2.99 1.39 0.75 0.56 0.60 2.84 2.69 1.34 3.02

Footnotes follow
Table A-2. Continued. Concentrations, ng/g (ppb) wet weight, of aromatic contaminants in stomach contents of juvenile chinook salmon sampled in 1990. - ^a,b,c,d,e

Site name
Commencement (City Waterway)
Method blanks
NIST reference tissue (Mytilus edulis)^j
Previously analyzed NIST tissue SRM 1974 (n = 124)

Date collected 6/26/90 6/28/90 6/28/90

Aromatic contaminants Lab no. 63-430 63-431^h 63-432^h 63-421 63-422 63-434 63-435 63-420 63-433 mean RSDⁱ

naphthalene
55
130
180
10
12
6
5
6
5
4
38

C1-naphthalenes 120 1200 1100 4 5 2 2 4 3 3 44

C2-naphthalenes 290 2700 3000 2 2 < 0.3 < 0.3 3 4 4 50

C3-naphthalenes 390 4500 5300 < 0.5 < 0.4 < 0.3 < 0.3 6 9 8 39

C4-naphthalenes 370 3600 4200 < 0.5 < 0.4 < 0.3 < 0.3 27 24 24 41

acenaphthylene 20 190 210 1 1 < 0.4 0.7 2 1 0.7 54

acenaphthene 78 4500 4500 < 0.7 < 0.6 < 0.5 < 0.5 0.9 1 0.8 37

fluorene 130 6800 7100 < 0.6 0.9 < 0.4 < 0.4 2 2 1 43

C1-fluorenes 140 2300 2500 < 0.6 < 0.5 < 0.4 < 0.4 6 6 4 31

C2-fluorenes 320 3100 3500 < 0.6 < 0.5 < 0.4 < 0.4 31 31 21 36

C3-fluorenes 360 2600 2600 < 0.6 < 0.5 < 0.4 < 0.4 65 42 24 61

phenanthrene 540 18000 18000 2 2 0.9 < 0.2 5 8 6 26

C1-phenanthrenes/anthracenes 470 5500 7200 < 0.3 1 < 0.2 < 0.2 9 9 11 33

C2-phenanthrenes/anthracenes 560 3500 4700 < 0.3 < 0.2 < 0.2 < 0.2 46 40 45 33

C3-phenanthrenes/anthracenes 670 2000 2800 < 0.3 < 0.2 < 0.2 < 0.2 60 52 49 35

C4-phenanthrenes/anthracenes 690 600 940 < 0.3 < 0.2 < 0.2 < 0.2 31 26 12 72

dibenzothiophene 49 2100 2200 < 0.3 0.4 < 0.2 < 0.2 1 1 0.9 38

C1-dibenzothiophenes 87 1700 1900 < 0.3 < 0.3 < 0.2 < 0.2 7 5 6 35

C2-dibenzothiophenes 250 2400 2500 < 0.3 < 0.3 < 0.2 < 0.2 39 35 30 34

C3-dibenzothiophenes 270 1800 1900 < 0.3 < 0.3 < 0.2 < 0.2 47 43 35 37

Sum of LACs 5900 69000 76000 19 24 9 8 400 350 270 40

fluoranthene 950 20000 24000 1 0.8 0.8 0.7 46 47 46 16

pyrene 770 11000 18000 0.8 0.6 0.7 0.5 44 46 44 16

C1-fluoranthenes/pyrenes 550 5100 9500 < 0.3 < 0.3 < 0.2 < 0.2 34 33 25 26

benz[a]anthracene 150 2900 7800 < 0.2 < 0.2 < 0.2 < 0.2 6 7 5 23

chrysene 330 2100 6800 < 0.2 < 0.2 0.4 0.4 18 20 18 14

C1-chrysenes/benz[a]anthracenes 220 900 4800 < 0.2 < 0.2 0.3 < 0.1 11 11 7 34

C2-chrysenes/benz[a]anthracenes 130 320 2000 < 0.2 < 0.2 < 0.1 < 0.1 5 6 3 70

C3-chrysenes/benz[a]anthracenes 110 130 810 < 0.2 < 0.2 < 0.1 < 0.1 3 3 1 84

C4-chrysenes/benz[a]anthracenes 31 30 140 < 0.2 < 0.2 < 0.1 < 0.1 < 0.1 0.3 0.6 89

benzo[b]fluoranthene 190 1400 4700 < 0.1 < 0.1 0.3 < 0.2 7 8 7 31

benzo[k]fluoranthene^f 170 1000 3900 < 0.1 < 0.1 0.3 < 0.2 5 6 4 28

benzo[a]pyrene 66 730 4100 < 0.1 < 0.1 < 0.2 < 0.1 2 3 2 51

indeno[1,2,3-cd]pyrene 76 420 3100 < 0.1 < 0.2 < 0.2 < 0.1 2 3 2 52

dibenz[a,h]anthracene 17 55 910 < 0.1 < 0.2 < 0.1 < 0.1 0.5 0.9 0.4 64

benzo[ghi]perylene 75 270 2200 < 0.1 < 0.2 < 0.2 < 0.1 3 6 2 44

Sum of HACs 3800 46000 93000 2 1 3 2 190 200 160 17

Sample weight, grams 0.71 1.52 2.33 3.02 3.00

Footnotes:

^a The concentrations of the analytes and the percent recoveries of the surrogate standards were calculated using hexamethylbenzene as the internal standard.

^b Concentrations and initial identifications were determined using gas chromatography/mass spectrometry (GC/MS) - multiple ion detection.

^c The "less than" symbol (<) indicates that the analyte was not detected in concentrations above the stated value.

^d Concentrations less than 10 are rounded to one significant figure; concentrations greater than 10 are rounded to two significant figures.

^e Percent recoveries for the internal standards (surrogates) averaged 91%, CV = 18%, n = 58. Percent recoveries of the surrogates include quality control samples.

^f Benzo[k]fluoranthene and benzo[j]fluoranthene often coelute. This combined concentration is reported.

^g Blank data were subtracted from the sample, and the results are reported here.

^h These samples are duplicate analyses. The data for the duplicate samples were somewhat different which implies that the samples were not homogeneous.

ⁱ RSD is the relative standard deviation which is the standard deviation divided by the mean and expressed as a percent.

^j Standard reference material from the National Institute of Science and Technology.

Table of Contents


	Site name	Green River Hatchery	Duwamish River estuary				Nisqually Hatchery		Nisqually River estuary^g			Puyallup Hatchery
	Date collected	5/8/90	5/24/90	6/1/90	6/18/90	5/29/90		6/13/90		6/14/90	6/4/90		6/6/90
Aromatic contaminants	Lab no.	63-411	63-413	63-414	63-415	63-417		63-428		63-429	63-418		63-419

naphthalene		26	51	230	2900	60		1		3	21		21
C1-naphthalenes		29	100	920	4200	42		3		5	18		28
C2-naphthalenes		34	120	1600	3500	35		1		2	25		54
C3-naphthalenes		40	170	3500	1900	47		0.8		1	30		53
C4-naphthalenes		43	170	3900	770	10		< 0.2		< 0.2	12		31
acenaphthylene		3	29	12	98	6		0.8		0.8	3		3
acenaphthene		2	76	86	8500	5		0.7		0.9	2		3
fluorene		4	99	220	9100	8		0.8		0.9	4		5
C1-fluorenes		9	75	990	1500	13		< 0.3		< 0.3	5		10
C2-fluorenes		20	210	3000	930	7		< 0.3		< 0.3	15		25
C3-fluorenes		< 0.3	240	2800	120	< 2		< 0.3		< 0.3	7		< 0.2
phenanthrene		18	440	870	20000	13		1		2	11		17
C1-phenanthrenes/anthracenes		37	360	2300	4700	11		1		1	15		25
C2-phenanthrenes/anthracenes		50	480	2500	2100	9		1		1	17		30
C3-phenanthrenes/anthracenes		65	380	2400	750	15		0.7		1	7		13
C4-phenanthrenes/anthracenes		17	150	990	210	< 1		< 0.1		< 0.1	< 0.3		8
dibenzothiophene		5	41	270	2300	2		< 0.3		< 0.3	2		4
C1-dibenzothiophenes		14	61	1200	690	3		0.5		0.3	6		12
C2-dibenzothiophenes		19	150	2600	450	3		1		0.4	8		15
C3-dibenzothiophenes		18	150	2300	230	< 1		0.8		0.8	7		13

Sum of LACs		450	3600	33000	65000	290		14		20	220		370

fluoranthene		4	1200	910	17000	7		2		1	6		7
pyrene		10	660	730	9200	7		1		1	6		7
C1-fluoranthenes/pyrenes		25	1200	1100	4900	9		2		0.6	4		7
benz[a]anthracene		2	520	210	2500	1		0.3		0.3	1		2
chrysene		4	1200	320	1900	2		2		0.6	2		3
C1-chrysenes/benz[a]anthracenes		10	340	410	810	1		0.4		0.3	1		5
C2-chrysenes/benz[a]anthracenes		10	100	350	220	< 0.6		< 0.1		< 0.1	< 0.2		1
C3-chrysenes/benz[a]anthracenes		7	36	260	55	< 0.6		< 0.1		< 0.1	< 0.2		1
C4-chrysenes/benz[a]anthracenes		0.6	< 0.2	110	< 0.5	< 0.6		< 0.1		< 0.1	< 0.2		< 0.1
benzo[b]fluoranthene		0.6	360	200	780	1		< 0.1		< 0.1	1		2
benzo[k]fluoranthene^f		0.4	310	130	670	0.8		0.2		0.2	0.8		0.7
benzo[a]pyrene		0.6	150	82	390	0.8		0.2		< 0.1	0.8		0.9
indeno[1,2,3-cd]pyrene		0.3	100	83	160	0.9		0.2		< 0.1	0.7		0.6
dibenz[a,h]anthracene		0.2	30	23	52	0.6		< 0.1		< 0.1	0.2		0.2
benzo[ghi]perylene		0.7	110	150	100	1		0.3		0.2	2		0.6

Sum of HACs		75	6300	5100	39000	32		9		4	26		38

Sample weight, grams		2.99	1.39	0.75	0.56	0.60		2.84		2.69	1.34		3.02

	Site name	Commencement (City Waterway)			Method blanks				NIST reference tissue (Mytilus edulis)^j			Previously analyzed NIST tissue SRM 1974 (n = 124)
	Date collected	6/26/90	6/28/90	6/28/90
Aromatic contaminants	Lab no.	63-430	63-431^h	63-432^h	63-421	63-422	63-434	63-435	63-420	63-433	mean	RSDⁱ

naphthalene		55	130	180	10	12	6	5	6	5	4	38
C1-naphthalenes		120	1200	1100	4	5	2	2	4	3	3	44
C2-naphthalenes		290	2700	3000	2	2	< 0.3	< 0.3	3	4	4	50
C3-naphthalenes		390	4500	5300	< 0.5	< 0.4	< 0.3	< 0.3	6	9	8	39
C4-naphthalenes		370	3600	4200	< 0.5	< 0.4	< 0.3	< 0.3	27	24	24	41
acenaphthylene		20	190	210	1	1	< 0.4	0.7	2	1	0.7	54
acenaphthene		78	4500	4500	< 0.7	< 0.6	< 0.5	< 0.5	0.9	1	0.8	37
fluorene		130	6800	7100	< 0.6	0.9	< 0.4	< 0.4	2	2	1	43
C1-fluorenes		140	2300	2500	< 0.6	< 0.5	< 0.4	< 0.4	6	6	4	31
C2-fluorenes		320	3100	3500	< 0.6	< 0.5	< 0.4	< 0.4	31	31	21	36
C3-fluorenes		360	2600	2600	< 0.6	< 0.5	< 0.4	< 0.4	65	42	24	61
phenanthrene		540	18000	18000	2	2	0.9	< 0.2	5	8	6	26
C1-phenanthrenes/anthracenes		470	5500	7200	< 0.3	1	< 0.2	< 0.2	9	9	11	33
C2-phenanthrenes/anthracenes		560	3500	4700	< 0.3	< 0.2	< 0.2	< 0.2	46	40	45	33
C3-phenanthrenes/anthracenes		670	2000	2800	< 0.3	< 0.2	< 0.2	< 0.2	60	52	49	35
C4-phenanthrenes/anthracenes		690	600	940	< 0.3	< 0.2	< 0.2	< 0.2	31	26	12	72
dibenzothiophene		49	2100	2200	< 0.3	0.4	< 0.2	< 0.2	1	1	0.9	38
C1-dibenzothiophenes		87	1700	1900	< 0.3	< 0.3	< 0.2	< 0.2	7	5	6	35
C2-dibenzothiophenes		250	2400	2500	< 0.3	< 0.3	< 0.2	< 0.2	39	35	30	34
C3-dibenzothiophenes		270	1800	1900	< 0.3	< 0.3	< 0.2	< 0.2	47	43	35	37

Sum of LACs		5900	69000	76000	19	24	9	8	400	350	270	40

fluoranthene		950	20000	24000	1	0.8	0.8	0.7	46	47	46	16
pyrene		770	11000	18000	0.8	0.6	0.7	0.5	44	46	44	16
C1-fluoranthenes/pyrenes		550	5100	9500	< 0.3	< 0.3	< 0.2	< 0.2	34	33	25	26
benz[a]anthracene		150	2900	7800	< 0.2	< 0.2	< 0.2	< 0.2	6	7	5	23
chrysene		330	2100	6800	< 0.2	< 0.2	0.4	0.4	18	20	18	14
C1-chrysenes/benz[a]anthracenes		220	900	4800	< 0.2	< 0.2	0.3	< 0.1	11	11	7	34
C2-chrysenes/benz[a]anthracenes		130	320	2000	< 0.2	< 0.2	< 0.1	< 0.1	5	6	3	70
C3-chrysenes/benz[a]anthracenes		110	130	810	< 0.2	< 0.2	< 0.1	< 0.1	3	3	1	84
C4-chrysenes/benz[a]anthracenes		31	30	140	< 0.2	< 0.2	< 0.1	< 0.1	< 0.1	0.3	0.6	89
benzo[b]fluoranthene		190	1400	4700	< 0.1	< 0.1	0.3	< 0.2	7	8	7	31
benzo[k]fluoranthene^f		170	1000	3900	< 0.1	< 0.1	0.3	< 0.2	5	6	4	28
benzo[a]pyrene		66	730	4100	< 0.1	< 0.1	< 0.2	< 0.1	2	3	2	51
indeno[1,2,3-cd]pyrene		76	420	3100	< 0.1	< 0.2	< 0.2	< 0.1	2	3	2	52
dibenz[a,h]anthracene		17	55	910	< 0.1	< 0.2	< 0.1	< 0.1	0.5	0.9	0.4	64
benzo[ghi]perylene		75	270	2200	< 0.1	< 0.2	< 0.2	< 0.1	3	6	2	44

Sum of HACs		3800	46000	93000	2	1	3	2	190	200	160	17

Sample weight, grams		0.71	1.52	2.33					3.02	3.00