Northwest Fisheries Science Center

Monster Seminar JAM

Event Information

Monster Seminar JAM - Using next generation sequencing to generate whole mitochondrial genomes for population genetics and phylogeography of cetaceans

Presentator(s):
Dr. Phillip Morin, Protected Resources Division, Southwest Fisheries Science Center

Long Description:
Killer whales (Orcinus orca) currently comprise a single, cosmopolitan species with a diverse diet. However, studies over the last 30 years have revealed populations of sympatric ecotypes with discrete prey preferences, morphology and behaviors. Although these ecotypes avoid social interactions and are not known to interbreed, genetic studies to date have found extremely low levels of diversity in the mitochondrial control region, and few clear phylogeographic patterns worldwide. This low level of diversity is likely due to low mitochondrial mutation rates that are common to cetaceans. Using killer whales as a case study, we have developed a method to readily sequence, assemble, and analyze complete mitochondrial genomes from large numbers of samples to more accurately assess phylogeography and estimate divergence times. This represents an important tool for wildlife management, not only for killer whales but for many marine taxa. We used high-throughput sequencing to survey whole mitochondrial genome variation of 139 samples from the North Pacific, North Atlantic and southern oceans. Phylogenetic analysis indicated that each of the known ecotypes represents a strongly supported clade with divergence times ranging from approximately 150,000 to 700,000 years ago. We recommend that three named ecotypes be elevated to full species, and that the remaining types be recognized as subspecies pending additional data. Establishing appropriate taxonomic designations will greatly aid in understanding the ecological impacts and conservation needs of these important marine predators. We predict that phylogeographic mitogenomics will become an important tool for improved statistical phylogeography and more precise estimates of divergence times, and discuss ongoing projects using similar technologies on blue, fin, sperm and killer whales, and leatherback turtles.

Location:
NWFSC  Map to NWFSC
2725 Montlake Blvd. E.
Seattle,  WA  98112

Date and Time:
Thursday, November 4, 2010, 11:00 am - 12:00 pm

Contact Person(s):
Tom Hom
206-860-3337
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