Northwest Fisheries Science Center

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Document Type: Journal Article
Center: NWFSC
Document ID: 1447
Title: Characteristics of paralytic shellfish toxins in seawater and sardines (Sardina pilchardus) during blooms of Gymnodinium catenatum
Author: Pedro R. Costa, Maria Joao Botelho, K. A. Lefebvre
Publication Year: 2010
Journal: Hydrobiologia
Volume: 655
Issue: 1
Pages: 89-97
Keywords: saxitoxins, paralytic shellfish poisoning, paralytic shellfish toxins, dissolved toxins, planktivorous fish,shellfish toxins
Abstract:

The re-emergence of Gymnodinum catenatum blooms after a 10 year hiatus of absence initiated the present investigation.  This study aims to evaluate the exposure of small pelagic fishes to paralytic shellfish toxins (PST) during blooms of G. catenatum.  Sardines (Sardina pilchardus) were selected as a representative fish species.  In order to assess toxin availability to fish, both intracellular PSTs (toxin retained within the algal cells) and extracellular PSTs (toxin found in seawater outside algal cells) were quantified, as well as toxin levels within three fish tissue matrices (viscera, muscle and brain).  During the study period, the highest cell densities of G. catenatum reached 2.5 × 104 cells l−1, and intracellular PST levels ranged from 3.4 to 398 ng STXeq l−1 as detected via an enzyme linked immunosorbent assay (ELISA).  Measurable extracellular PSTs were also detected in seawater (0.2–1.1 μg STXeq l−1) for the first time in Atlantic waters.  The PST profile in G. catenatum was determined via high performance liquid chromatography with fluorescence detection (HPLC-FLD) and consisted mostly of sulfocarbamoyl (C1+2, B1) and decarbamoyl (dcSTX, dcGTX2+3, dcNEO) toxins.  The observed profile was similar to that reported previously in G. catenatum blooms in this region before the 10-year hiatus.  Sardines, planktivorous fish that ingest a large number of phytoplankton cells, were found to contain PSTs in the viscera, reaching a maximum of 531 μg STXeq kg−1.  PSTs were not detected in corresponding muscle or brain tissues.  The PST profile characterized in sardine samples consisted of the same sulfocarbamoyl and decarbamoyl toxins found in the algal prey with minor differences in relative abundance of each toxin.  Overall, the data suggest that significant biotransformation of PSTs does not occur in sardines.  Therefore, planktivorous fish may be a good tracer for the occurrence of offshore G. catenatum blooms and the associated PSTs produced by these algae.

URL1: The next link will exit from NWFSC web site http://dx.doi.org/10.1007/s10750-010-0406-5
Theme: Sustainable, safe and secure seafood for healthy populations and vibrant communities
Foci: Provide scientific support to ensure safe seafood for healthy populations and characterize how human activities and climate affect risks from pathogens, chemical contaminants and biotoxins