|Document Type:||Journal Article|
|Title:||Microsatellite allele ladder-based standardization: an application in Oncorhynchus tshawytscha|
|Author:||E. LaHood, O. Schlei, J. K. Wenburg, Paul Moran|
|Journal:||Molecular Ecology Resources|
|Keywords:||GAPS, allele ladders, standardization, genotyping, microsatellite,microsatellite DNA,allele ladder,standardization,inter-laboratory,genotyping|
Interagency collaborations using a common set of microsatellite loci require a standard allele naming system, which can be challenging effort and cost. In contrast to commercial breeding or forensics, the challenge to standardize is especially acute for ecological genetics collaborations. In particular, allele standardization in salmonid and other species continues to involve the burdensome task of distributing many individual DNA samples and performing several hundred to thousands of PCR amplifications to accommodate large numbers of alleles. In Oncorhynchus tshawytscha), we applied an allele ladder-based standardization approach to reduce the effort associated with alleles throughout the species range.
In Oncorhynchus tshawytscha), we applied an allele ladder-based standardization approach to reduce the effort associated with alleles.
LaHood, E., O. Schlei, J. Wenburg and P. Moran 2012. Microsatellite allele ladder-based standardization: an application in Oncorhynchus tshawytscha. Molecular Ecology Resources. Vol. 12, pp. 185-189. DOI: 10.1111/j.1755-0998.2011.03088.x