Northwest Fisheries Science Center

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Document Type: Journal Article
Center: NWFSC
Document ID: 1626
Title: Microsatellite allele ladder-based standardization: an application in Oncorhynchus tshawytscha
Author: E. LaHood, O. Schlei, J. K. Wenburg, Paul Moran
Publication Year: 2012
Journal: Molecular Ecology Resources
Volume: 12
Issue: 1
Pages: 185-189
DOI: 10.1111/j.1755-0998.2011.03088.x
Keywords: GAPS, allele ladders, standardization, genotyping, microsatellite,microsatellite DNA,allele ladder,standardization,inter-laboratory,genotyping
Abstract:

Interagency collaborations using a common set of microsatellite loci require a standard allele naming system, which can be challenging due to effort and cost. In contrast to commercial breeding or forensics, the challenge to standardize is especially acute for ecological genetics collaborations. In particular, allele standardization in salmonids and other species continues to involve the burdensome task of distributing many individual DNA samples and performing several hundred to thousands of PCR amplifications to accommodate large numbers of alleles. In Chinook salmon (Oncorhynchus tshawytscha), we applied an allele ladder-based standardization approach to reduce the effort associated with standardizing laboratories to recognize and identify a common set of alleles throughout the species range.

Description:

In Chinook salmon (Oncorhynchus tshawytscha), we applied an allele ladder-based standardization approach to reduce the effort associated with standardizing laboratories to recognize and identify a common set of alleles throughout the species range.

Official Citation:

LaHood, E., O. Schlei, J. Wenburg and P. Moran 2012. Microsatellite allele ladder-based standardization: an application in Oncorhynchus tshawytscha. Molecular Ecology Resources. Vol. 12, pp. 185-189. DOI: 10.1111/j.1755-0998.2011.03088.x