|Document Type:||Journal Article|
|Title:||The sablefish (Anoplopoma fimbria) karyotype including the location of 5S and 18S rDNA and information on cell culture conditions|
|Author:||R. B. Phillips, Joshua Faber-Hammond, J. A. Luckenbach|
Sablefish Anoplopoma fimbria is a long-lived groundfish found in the North Pacific Ocean which is now being successfully cultured in the United States and Canada. A genetic map utilizing microsatellite markers was recently prepared for this species, and there is considerable interest in ploidy manipulation to produce sterile or monosex sablefish populations for aquaculture. Since nothing was previously known about the chromosomes or ploidy of sablefish, this study focused on developing methods for culturing white blood cells to obtain chromosomes and describe the karyotype of sablefish. In this paper, we present the first information on the karyotype of this species including the location of the 5S and 18S rDNA loci as determined by fluorescence in situ hybridization (FISH) and describe our methods for blood culture.