Northwest Fisheries Science Center

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Document Type: Journal Article
Center: NWFSC
Document ID: 4193
Title: Endocrine Disrupting Chemicals in Fish Bile: A Rapid Method of Analysis Using English Sole (Parophrys vetulus) from Puget Sound, WA, USA
Author: Denis A.M. da Silva, J. Buzitis, W. L. Reichert, J. E. West, Sandra M. O'Neill, Lyndal L. Johnson, T. K. Collier, G. M. Ylitalo
Publication Year: 2013
Journal: Chemosphere
Volume: 92
Issue: 11
Pages: 1550 - 1556
Keywords: endocrine disruptors, bisphenol A, 17²-estradiol, 17±-ethynylestradiol, bile, Puget Sound ,

This study describes a recently developed and rapid method to measure bisphenol A (BPA), 17b-estradiol (E2) and 17a-ethynylestradiol (EE2) in bile of fish using ultra-performance liquid chromatography with tandem mass spectrometry. The limits of quantitation (LOQ) for BPA, EE2 and E2 were 6.3ng/mL, 12.5ng/mL and 6.3ng/mL, respectively. These compounds were analyzed in bile of male English sole (Parophrys vetulus) collected from urban and non-urban sites in Puget Sound, WA, USA. The BPA and E2 concentrations (and occurrence) ranged from < LOQ - 52 ng/mL (10-100%) and < LOQ - 310 ng/mL (10-70%), respectively. EE2 levels were below the LOQ in all samples. Urban sites were significantly different than non-urban site, demonstrating the environmental applicability of this analytical method. Moreover, this study presented, for the first time in United States coastal waters, environmental concentrations of these compounds in male English sole collected from Puget Sound, a region of increasing urbanization.


The study described a new method of simultaneous analysis of important endocrine disrupting chemicals such as BPA, EE2 and E2 in fish bile samples. It also include a field validation using English sole bile from Puget Sound.

Theme: Sustaining Marine Ecosystem and Human Health
Foci: Develop methods, technologies, and data integration tools to predict ocean-related public health risks into health early warning and ocean observing systems