Northwest Fisheries Science Center

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Document Type: Journal Article
Center: NWFSC
Document ID: 4358
Title: Identification of ovarian genes regulated by follicle-stimulating hormone (Fsh) in vitro during early secondary oocyte growth in coho salmon
Author: J. A. Luckenbach, Yoji Yamamoto, Jose Maria Guzman, P. Swanson
Publication Year: 2013
Journal: Molecular and Cellular Endocrinology
Volume: 366
Pages: 38-52
Keywords: Follicle-stimulating hormone,Ovarian follicle,Subtractive hybridization,Gene expression,Granulosa cells,Fish,

Follicle-stimulating hormone (Fsh) function in fishes is poorly understood. This study aimed to reveal
Fsh-regulated genes in coho salmon previtellogenic ovarian follicles in vitro. Four suppression subtractive
hybridization libraries were generated with RNA isolated from Fsh-treated and control follicles or follicle
cell-enriched tissue fractions. Fsh induced steroidogenesis and dynamically upregulated several genes
predominantly expressed in follicle cells, including WAP domain-containing protease, connexin 34.3, clusterin
(clu1, clu2), fibronectin, wilms tumor 2-like, and influenza virus NS1A-binding protein a. Genes downregulated
by Fsh included connective tissue growth factor, alcohol dehydrogenase 8-like, and serine/
threonine-protein kinase pim-1. This study demonstrates for the first time in fishes that Fsh influences
the expression of a unique suite of ovarian genes involved in processes like cell communication, survival
and differentiation, and extracellular matrix remodeling. Collectively, these findings suggest that Fsh and/
or steroids induce differentiation of granulosa cells and remodeling of the follicle in preparation for onset
of vitellogenesis.

Theme: Recovery, Rebuilding and Sustainability of Marine and Anadromous Species