Northwest Fisheries Science Center

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Document Type: Journal Article
Center: NWFSC
Document ID: 4375
Title: Impact of stirring on the luminescent optical dissolved oxygen optode
Author: John Edwin Colt
Publication Year: 2014
Journal: North American Journal of Aquaculture
Volume: 76
Pages: 242-244
DOI: 10.1080/15222055.2014.893474
Keywords: dissolved oxygen,luminescent optode,stirring,

 The impact of stirring on the response of the YSI ProODO luminescence dissolved oxygen sensor or optode was measured in a 250 mL BOD with a magnetic stirrer on or off. With a water sample from a simulated fish transport experiment, the dissolved oxygen dropped by 2-3 mg L-1 when the magnetic stirrer was turned off for 11 minute. The dissolved oxygen returned to a value projected from the oxygen uptake rate when the stirrer was turned on. A much smaller impact was observed for 18.2 M high purity water (mean: -0.11 mg L-1). Acid washing the BOD bottle/stir bar and cleaning the optode cap reduced the decrease to -0.08 mg L-1. Based on t-tests between pairs of stirring-on and stirring-off, the lack of stirring had a significant impact on measured dissolved oxygen. The source of this effect and its potential impact on the accuracy of the optical probe under typical operating conditions remains to be determined.

Theme: Recovery, Rebuilding and Sustainability of Marine and Anadromous Species
Foci: Maximize effectiveness and minimize impacts of artificial propagation in recovery, rebuilding and stock sustainability