|Document Type:||Journal Article|
|Title:||Zebrafish as a model for Vibrio parahaemolyticus virulence|
|Author:||R. N. Paranjpye, Mark S. Myers|
|Keywords:||Vibrio parahaemolyticus, virulence, zebrafish,Vibrio parahaemolyticus,virulence,Zebrafish,|
Vibrio parahaemolyticus, is a Gram-negative, naturally occurring marine bacterium. Subpopulations of strains belonging to this species cause an acute self-limiting gastroenteritis in humans, and less commonly, wound infections. In vivo models to differentiate avirulent and virulent strains and evaluate the pathogenic potential of strains of this species have been largely focused on evaluating the effects of known virulence factors such as thermostable direct hemolysin (TDH), the thermolabile related hemolysin (TRH) or the contributions of the type 3 secretion systems (T3SSs). However, virulence is likely to be multifactorial and additional, and yet to be identified factors likely contribute to virulence in this bacterium. In this study we investigated an adult zebrafish model to assess the overall virulence of V. parahaemolyticus strains. The model could detect differences in the virulence potential of strains when animals were challenged intraperitoneally, based on survival time. Differences in survival were noted irrespective of the source of isolation of the strain (environmental or clinical) and regardless of the presence or absence of the known virulence factors TDH and TRH, suggesting the influence of additional virulence factors. The model was also effective in comparing differences in virulence between the wild-type V. parahaemolyticus strain RIMD2210633 and isogenic pilin mutants DpilA and D
|Theme:||Sustaining Marine Ecosystem and Human Health|
Develop methods, technologies, and data integration tools to predict ocean-related public health risks into health early warning and ocean observing systems
Ensure safe and sustainable seafood for healthy populations.
Paranjpye, Rohinee, Mark Meyers, Evan Yount and Jesssica Thompson. 2012. Zebrafish as a model for Vibrio parahaemolyticus virulence. Infection and Immunity.