Northwest Fisheries Science Center

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Document Type: Journal Article
Center: NWFSC
Document ID: 4492
Title: Expression of Duplicate msa Genes in the Salmonid Pathogen Renibacterium salmoninarum
Author: L. D. Rhodes, A. M. Coady, M. S. Strom
Publication Year: 2002
Journal: Applied and Environmental Microbiology
Volume: 68
Issue: 11
Pages: 5480-5487
DOI: 10.1128/AEM.68.11.54805487.2002
Keywords: bacterial kidney disease

Renibacterium salmoninarum is a gram-positive bacterium responsible for bacterial kidney disease of salmon and trout. R. salmoninarum has two identical copies of the gene encoding major soluble antigen (MSA), an immunodominant, extracellular protein. To determine whether one or both copies of msa are expressed, reporter plasmids encoding a fusion of MSA and green fluorescent protein controlled by 0.6 kb of promoter region from msa1 or msa2 were constructed and introduced into R. salmoninarum. Single copies of the reporter plasmids integrated into the chromosome by homologous recombination. Expression of mRNA and protein from the integrated plasmids was detected, and transformed cells were fluorescent, demonstrating that both msa1 and msa2 are expressed under in vitro conditions. This is the first report of successful transformation and homologous recombination in R. salmoninarum.

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Theme: Recovery, Rebuilding and Sustainability of Marine and Anadromous Species
Foci: Maximize effectiveness and minimize impacts of artificial propagation in recovery, rebuilding and stock sustainability