Northwest Fisheries Science Center

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Document Type: Journal Article
Center: NWFSC
Document ID: 4493
Title: Sensitive detection of Renibacterium salmoninarum in whole fry, blood, and other tissues of Pacific salmon by reverse transcriptionpolymerase chain reaction
Author: L. D. Rhodes, W. B. Nilsson, M. S. Strom
Publication Year: 1998
Journal: Molecular Marine Biology and Biotechnology
Volume: 7
Issue: 4
Pages: 270-279
Keywords: bacterial kidney disease

 A sensitive, reproducible assay for detecting Renibacterium salmoninarum in a variety of tissues, in- cluding blood, has been developed. This assay, based on reverse transcription–polymerase chain reaction (RT-PCR) of 16S ribosomal RNA, exhibited sensitivity to 10 cells per milligram of tissue by ethidium bromide detection, and this sensitivity was increased 10-fold by Southern blotting. There was a strong association (p < .001) between blood and ovarian fluid for the presence of bacteria in spawning salmon, and in 17.9% of the infected fish, bacteria were detected in blood but not in ovarian fluid. The ability to analyze multiple tis- sues, the reproducibility, and sensitivity of 16S RT- PCR make it a useful tool for both research and husbandry applications.

Theme: Recovery, Rebuilding and Sustainability of Marine and Anadromous Species
Foci: Maximize effectiveness and minimize impacts of artificial propagation in recovery, rebuilding and stock sustainability