Northwest Fisheries Science Center

Display All Information

Document Type: Journal Article
Center: NWFSC
Document ID: 4494
Title: Description and characterization of IS994, a putative IS3 family insertion sequence from the salmon pathogen, Renibacterium salmoninarum
Author: L. D. Rhodes, T. Hilton Grayson, S. M. Alexander, M. S. Strom
Publication Year: 2000
Journal: Gene
Volume: 244
Issue: 1-2
Pages: 97-107
DOI: 10.1016/S0378-1119(99)00573-9,
Keywords: bacterial kidney disease,Genetics
Abstract:

Renibacterium salmoninarum, a slowly growing, Gram-positive bacterium, is responsible for bacterial kidney disease in salmonid fishes world-wide. To date, no mobile genetic elements have been reported for this pathogen. Here, we describe the first insertion sequence (IS) identified from R. salmoninarum. This element, IS994, has a significant predicted amino acid sequence homology (64.8 and 71.9%) to the two open reading frames encoding the transposase of IS6110 of Mycobacterium tuberculosis. Protein parsimony and protein distance matrix analyses show that IS994 is a member of group IS51 of the IS3 family. From a conservative estimate, there are at least 17 chromosal insertions of IS994 or closely related elements. Sequence analysis of seven of these loci reveals single nucleotide polymorphisms throughout the element (including the terminal inverted repeats), a 15 bp insertion in three of the seven loci, and an absence of flanking direct repeats or conserved insertion site. Restriction fragment length polymorphism analysis of XbaI-digested chromosomal DNA shows variations among European and North American isolates, indicating that IS994 may be a useful molecular marker for epizootiological studies.

URL1: The next link will exit from NWFSC web site http://www.sciencedirect.com/science/article/pii/S0378111999005739
Theme: Recovery, Rebuilding and Sustainability of Marine and Anadromous Species
Foci: Maximize effectiveness and minimize impacts of artificial propagation in recovery, rebuilding and stock sustainability