Northwest Fisheries Science Center

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Document Type: Journal Article
Center: NWFSC
Document ID: 4507
Title: Molecular characterization and quantification of sablefish (Anoplopoma fimbria) gonadotropins and their receptors: Reproductive dysfunction in female captive broodstock
Author: Jose Maria Guzman, J. A. Luckenbach, P. Swanson
Publication Year: 2013
Journal: General and Comparative Endocrinology
Volume: 193
Pages: 37-47
DOI: http://dx.doi.org/10.1016/j.ygcen.2013.07.007
Keywords: Anoplopoma fimbria,gonadotropin,gonadotropin receptor,reproductive dysfunction,sablefish,
Abstract:

Efforts to establish an aquaculture industry for sablefish (Anoplopoma fimbria) are constrained by reproductive dysfunction in wild-caught fish and by lack of reproduction of F1 females. Toward a better understanding of the reproductive dysfunction of captive broodstock, full-length cDNAs encoding the sablefish gonadotropin subunits (fshb, lhb and cga) and their receptors (fshr and lhcgr) were cloned, sequenced and quantitative real-time PCR assays developed. Sablefish gonadotropin subunits display some unique features, such as two additional Cys residues in the N-terminal region of Fshb and a lack of potential N-glycosylation sites in Fshb and Lhb, whereas Fshr and Lhcgr possess conserved structural characteristics described in other vertebrates. Wild females captured in fall completed gametogenesis in captivity the next spawning season, whereas females captured three months earlier, during summer, failed to mature. Interestingly, these wild non-maturing females exhibited similar reproductive features as prepubertal F1 females, including low levels of pituitary gonadotropin and ovarian receptor mRNAs and plasma sex steroids, and ovarian follicles arrested at the perinucleolus stage. In conclusion, this study described the cloning, molecular characterization and development of qPCR for sablefish gonadotropins and their receptors. Rearing conditions may impair vitellogenic growth of ovarian follicles in sablefish, compromising the reproductive success of broodstock.

Theme: Recovery, Rebuilding and Sustainability of Marine and Anadromous Species
Foci: Investigate ecological and socio-economic effects of alternative management strategies or governance structures.