|Document Type:||Journal Article|
|Title:||Vibrio ordalii sp. nov.: A causative agent of vibriosis in fish|
|Author:||Michael H. Schiewe, Trevor J. Trust, Jorge H. Crosa|
Vibrio ordalii sp. nov. is the name proposed for the bacterium previously designated as V. anguillarum biotype 2. The change in the classification of this fish pathogen is based on differences between the classical V. anguillarum and V. ordalii in cultural and biochemical characteristics, and in deoxyribonucleic acid (DNA) sequence relatedness. Phenotypically,V. ordalii was distinguishable from V. anguillarum based on: negative Voges-Proskauer reaction; negative reaction with arginine in Moeller's medium; negative Simmons' and Christensen's citrate test; negative ONPG test; failure to hydrolyze starch; failure to show lipase activity; inability to grow at 37°C; and failure to ferment cellobiose, glycerol, sorbitol, and trehalose. Genotypically, strain of V. ordalii formed a highly conserved DNA homology group which showed 83 to 100% within-group homology and only 58 to 69% relatedness to V. anguillarum. In contrast, the V. anguillarum strains tested showed greater than 70% withingroup homology and 53 to 67% relatedness to V. ordalii. Neither V. ordalii nor V. anguillarum were related to V. parahaemolyticus or V. alginolyticus. The proposed type strain (holotype) of V. ordalii is ATCC 33509 (=DF3K=Dom F3 kid).