Northwest Fisheries Science Center

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Document Type: Journal Article
Center: NWFSC
Document ID: 7670
Title: Evidence for colonization and destruction of hinge ligaments in cultured juvenile Pacific oysters (Crassostrea gigas) by cytophaga-like bacteria
Author: Christopher F. Dungan, Ralph A. Elston, Michael H. Schiewe
Publication Year: 1989
Journal: Applied and Environmental Microbiology
Volume: 55
Issue: 5
Pages: 1128-1135
Abstract:

Several strains of cytophaga–like gliding bacteria (CLB) were isolated as numerically dominant or codominant components of bacterial populations associated with proteinaceous hinge ligaments of cultured juvenile Pacific oysters, Crassostrea gigas.  These bacteria were morphologically similar to long, flexible bacilli occurring within degenerative lesions in oyster hinge ligaments.  Among bacteria isolated from hinge ligaments, only CLB strains were capable of sustained growth with hinge ligament matrix as the sole source of organic carbon and nitrogen.  In vitro incubation of cuboidal portions of ligament resilium with ligament CLB resulted in bacterial proliferation on the surfaces and penetration deep into ligament matrices.  Bacterial proliferation was accompanied by loss of resilium structural and mechanical integrity, including complete liquefaction, at incubation temperatures between 10 and 20°C.  The morphological, distributional, and degradative characteristics of CLB isolated from oyster hinge ligaments provide compelling, albeit indirect, evidence that CLB are the agents of a degenerative disease affecting juvenile cultured oysters.  The motility, metabolic, and hydrolytic characteristics of hinge ligament CLB and the low moles percent G + C values (32.4 to 32.9) determined for three representative strains indicate that they are marine Cytophaga spp. 

URL1: The next link will exit from NWFSC web site http://www.ncbi.nlm.nih.gov/pmc/articles/PMC184265/
Notes: Article has no doi but is available free on PubMed Central, and NIH website: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC184265/