Northwest Fisheries Science Center

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Document Type: Journal Article
Center: NWFSC
Document ID: 841
Title: A centralized model for creating shared, standarized, microsatellite data that simplifies inter-laboratory collaboration
Author: J. J. Stephenson, M. R. Campbell, J. E. Hess, Christine C. Kozfkay, A. P. Matala, M. V. McPhee, Paul Moran, S. R. Narum, M. M. Paquin, O. Schlei, M. P. Small, Donald M. Van Doornik, J. K. Wenburg
Publication Year: 2008
Journal: Conservation Genetics
Volume: 10
Issue: 4
Pages: 1145-1149
Keywords: microsatellites, standardization, steelhead, Oncorhynchus mykiss

We demonstrate an efficient model for standardizing microsatellite DNA data among laboratories studying Oncorhynchus mykiss.  Eight laboratories standardized 13 microsatellite loci following allele nomenclature of a central laboratory (average inter-laboratory genotyping concordance >98%).  Following this central model, we have currently standardized 298 alleles from throughout the species native range.  Although we focus here on O. mykiss, our experiences and recommendation apply equally to other broadly distributed species that may benefit from multi-laboratory collaborative data collection. 

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