Northwest Fisheries Science Center

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Document Type: Journal Article
Center: NWFSC
Document ID: 8467
Title: The thermostable direct hemolysin-related hemolysin (trh) gene for Vibrio parahaemolyticus: Sequence variation and implications for detection and function
Author: William B. Nilsson, Jeffrey W. Turner
Publication Year: 2016
Journal: Journal of Microbiological Methods
Volume: 126
Pages: 1-7
DOI: 10.1016/j.mimet.2016.04.007
Keywords: V. parahaemolyticus, virulence markers, tdh-ralated hemolysin, sequence variation, detection,
Abstract:

Vibrio parahaemolyticus is a leading cause of bacterial food-related illness associated with the consumption of undercooked seafood. Only a small subset of strains is pathogenic.  Most clinical strains encode for the thermostable direct hemolysin (TDH) and/or the TDH-related hemolysin (TRH). In this work, we amplify and sequence the trh gene from 80 trh+ strains of this bacterium and identify thirteen genetically distinct alleles, most of which have not been deposited in GenBank previously. Sequence data was used to design new primers for more reliable detection of trh by endpoint PCR. We also designed a new quantitative PCR assay to target a more conserved gene that is genetically-linked to trh. This gene, ureR, encodes the transcriptional regulator for the urease gene cluster immediately upstream of trh. We propose that this ureR assay can be a useful screening tool as a surrogate for direct detection of trh that circumvents challenges associated with trh sequence variation.

Theme: Sustainable, safe and secure seafood for healthy populations and vibrant communities
Foci: Provide scientific support to ensure safe seafood for healthy populations and characterize how human activities and climate affect risks from pathogens, chemical contaminants and biotoxins
Provide scientific support to ensure safe seafood for healthy populations and characterize how human activities and climate affect risks from pathogens, chemical contaminants and biotoxins