Northwest Fisheries Science Center

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Document Type: Journal Article
Center: NWFSC
Document ID: 8620
Title: Microsatellite markers for population genetic applications in the domoic acid-producing diatom Pseudo-nitzschia australis (Bacillariophyceae)
Author: N. G. Adams, P. L. Schwenke, G. Jason Smith, Vera L. Trainer
Publication Year: 2017
Journal: Protist
Volume: 168
Pages: 197-205
DOI: 10.1016/j.protis.2017.01.002
Keywords: Pseudo-nitzschia australis,microsatellite DNA,domoic acid,Population genetics

Microsatellites are commonly used markers in population genetics and are increasingly being employed to determine population structure in phytoplankton populations.  We have developed seven polymorphic microsatellite markers for the domoic-acid producing diatom Pseudo-nitzschia australis.  Using these markers, thirty P. australis isolates were genotyped, 10 isolates were from Monterey Bay, California and 20 were from off the northern coast of Oregon.  The number of alleles per locus ranged from two to eight and observed heterozygosities ranged from 0.11 to 0.70.  All but two of the isolates were genetically distinct and initial population differentiation analysis indicated no significant differences between the Pacific Northwest isolates and the Monterey Bay isolates.  Pseudo-nitzschia australis microsatellites appear species specific based on cross amplification tests with Pseudo-nitzschia fraudulenta, Pseudo-nitzschia seriata, Pseudo-nitzschia pungens and Pseudo-nitzschia multiseries.


An article describing the development of microsatellite markers for the domoic acid-producing diatom Pseudo-nitzschia australis.

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Theme: Sustainable, safe and secure seafood for healthy populations and vibrant communities
Foci: Provide scientific support to ensure safe seafood for healthy populations and characterize how human activities and climate affect risks from pathogens, chemical contaminants and biotoxins
Official Citation:

Adams NG, Schwenke P, Smith GJ, Trainer VL (2017) Microsatellite Markers for Population Genetic Applications in the Domoic Acid-producing Diatom Pseudo-nitzschia australis Frenguelli (Bacillariophyceae). Protist 168: 197-205