Northwest Fisheries Science Center

Project Meetings

First Annual MERHAB: Azaspiracid and Diarrhetic Shellfish Project Team Meeting

All project participants gave their presentations as scheduled (see Appendix 1, below). Because WA and MA have been tentatively approved for shellfish export to the EU – Spain and the Netherlands - (because these States have the most well developed toxin testing programs), monitoring for YTX, PTX and AZA will become increasingly important (see The next link/button will exit from NWFSC web site http://issuu.com/eastcoastshellfish/docs/2016dcnewsletter/3?e=3708857/34089939) John Kim (visiting scientist from Hanyang University, Seoul, Korea) has potentially isolated a new Azadinium species. This organism will be cultured in order to do genetic and morphological (SEM) characterization.

Catherine Sloan reported on the lower yields for AZA analysis. Further work will be done to improve these yields.

This project provides training to local partners as well as developing nations in the development of effective monitoring programs for harmful algae as well as their biotoxins in seafood and seawater. To date, training classes have been held for Puget Sound partners (SoundToxins) and outer coast Olympic Region Harmful Algal Blooms (ORHAB) collaborators as well as for government agencies in the Philippines, Indonesia and Guatemala as part of the North Pacific Marine Science Organization's (PICES) program on Seafood Safety in Developing Nations. A training class is currently being planned for India in 2014.

Greg Doucette and Zhihong Wang summarized NOAA Charleston LC-MS/MS efforts, emphasizing salt and matrix contamination and how to minimize via hardware set-up and LC elution programming; advantages (e.g., incr. sensitivity) of alkaline vs. acidic mobile phases discussed; need for spike-recovery studies of shellfish matrices highlighted.

Summary Tasks Resulting from the Workshop

  1. November 18, 2015. Project partners met in Long Beach, WA, at the National Conference on Harmful Algae in the U.S. to discuss project logistics. Meeting notes can be found here.
  2. January 21-22, 2016. Whitney Stutts from the US FDA visited Seattle to discuss LC/MS methods and provide training. Notes of her visit can be found here.
  3. March 18, 2016. Project partners met…The meeting summary and agenda can be found here. The 6 sample sites were chosen based on previous detection of lipophilic toxins and the importance of shellfish harvesting in the area. The meeting agenda can be found here, all presentations and a map of the sample sites can be found here.
  4. March 8-10, 2017. Project partners held a discussion at the Washington State Department of Health lab in Shoreline, held their annual meeting at the Jamestown S'Klallam Tribe center in Sequim Bay, WA, and met with the Washington State Department of Wildlife and attended a razor clam dig. The meeting agenda can be found here and photos from the meetings can be found here.