Northwest Fisheries Science Center

First Annual MERHAB: Azaspiracid and Diarrhetic Shellfish Project Team Meeting

All project participants gave their presentations as scheduled (see Appendix 1, below). Because WA and MA have been tentatively approved for shellfish export to the EU – Spain and the Netherlands - (because these States have the most well developed toxin testing programs), monitoring for YTX, PTX and AZA will become increasingly important (see The next link/button will exit from NWFSC web site http://issuu.com/eastcoastshellfish/docs/2016dcnewsletter/3?e=3708857/34089939) John Kim (visiting scientist from Hanyang University, Seoul, Korea) has potentially isolated a new Azadinium species. This organism will be cultured in order to do genetic and morphological (SEM) characterization.

Catherine Sloan reported on the lower yields for AZA analysis. Further work will be done to improve these yields.

This project provides training to local partners as well as developing nations in the development of effective monitoring programs for harmful algae as well as their biotoxins in seafood and seawater. To date, training classes have been held for Puget Sound partners (SoundToxins) and outer coast Olympic Region Harmful Algal Blooms (ORHAB) collaborators as well as for government agencies in the Philippines, Indonesia and Guatemala as part of the North Pacific Marine Science Organization's (PICES) program on Seafood Safety in Developing Nations. A training class is currently being planned for India in 2014.

Greg Doucette and Zhihong Wang summarized NOAA Charleston LC-MS/MS efforts, emphasizing salt and matrix contamination and how to minimize via hardware set-up and LC elution programming; advantages (e.g., incr. sensitivity) of alkaline vs. acidic mobile phases discussed; need for spike-recovery studies of shellfish matrices highlighted.

Summary Tasks Resulting from the Workshop